Isolation and identification of trophoblast lymphocyte cross-reactive (TLX) antigens from human lymphocytes

Isolation and identification of trophoblast lymphocyte cross-reactive (TLX) antigens from human lymphocytes.

It has been proposed that allotypic trophoblast lymphocyte cross-reactive (TLX) antigens are involved in the maintenance of normal human reproduction. Despite such a potentially important role for TLX antigens, isolation of human TLX proteins has not yet been reported. As an initial step toward elucidation of the structure and function of TLX antigens, we have isolated TLX proteins from Lubrol-...

Antigens of human trophoblast. Effects of heterologous anti-trophoblast sera on lymphocyte responses in vitro

This report describes the inhibition of human mixed lymphocyte culture (MLC) reactions by rabbit antisera to intact and detergent solubilized, fractionated, human trophoblast membranes. Heat-inactivated antisera were passed through solid-phase immunoabsorption columns of normal human serum and extensively absorbed with human erythrocytes, lymphocytes and liver powder. Immunohistological experim...

Isolation of human lymphocyte antigens class I-restricted cytotoxic T lymphocytes against autologous myeloma cells.

Peripheral blood T cells from a patient with multiple myeloma in complete remission were selected in vitro against an autologous myeloma cell line (SBN-1), using a protocol designed for the selection of relatively rare precursor cytotoxic T cells (pCTL). Delayed addition (2 weeks) of interleukin 2 induced T-cell proliferation, and a bulk culture (T-cell line) was obtained 2 days later. This T-c...

Isolation and identification of excretory-secretory and somatic antigens from the Oestrus ovis larvae by SDS-PAGE and immunoblotting

Oestrus ovis is an economically important parasite of small ruminants and a zoonotic parasite with many reports of ophthalmomyiasis in human from Iran and other countries. The aim of the peresent studywas the isolation and identification of excretory-secretory (ES) and somatic (S) antigens of O. ovis second and third stage larvae (L2, L3) collected from Arabi sheep breeds loca...

Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii Running title Cross-reactive antigens between N. caninum and T. gondii Authors